QUANTITATIVE DETERMINATION OF ETORICOXIB AND PARACETAMOL IN PHARMACEUTICAL DOSAGE FORM AND IN-VITRO COMPARISON BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC)
The objective of this present work was to
develop and validate analytical method for quantitative
determination of Paracetamol and Etoricoxib in a tablet
formulation and also the comparison of invitro data with
reference dosage form. Chromatographic separations of the
two drugs were analyzed on a Kromasil C18 column (25cm
X 4.6mm, 5 m). The mobile phase constituted of Buffer:
Acetonitirile with gradient program was delivered at the
flow rate 1.0 mL/min. Detection was performed at 220 nm.
Separation was completed within 20 min. Calibration
curves were linear with coefficient correlation between
0.99 to 1.0 over a concentration range of 48 to 146 g/mL
of Paracetamol and 6 to 19 g/mL for Etoricoxib
respectively. The relative standard deviation (R.S.D) was
found to be less than 2.0%. Analysis for dissolution study
was also performed by Reversed-Phase High Performance
Liquid Chromatography (RP-HPLC) method. Difference
factor (f1) were found to be 2.85 and 3.83 and similarity
factor (f2) were found to be 73.514 and 68.961 for
Paracetamol and Etoricoxib respectively.
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