ESTIMATION OF GATIFLOXACIN IN PHARMACEUTICAL DOSAGE FORMS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A. LAKSHMANA RAO, B.N.V. RAVI KUMAR, G.GIRIJA SANKAR

Abstract


A simple, rapid, sensitive and precise
High Performance Liquid Chromatographic
(HPLC) method has been developed for the
estimation of gatifloxacin in its pharmaceutical
dosage forms. In this method RP-C18 column
(150mmx4.6mm I.D., 5 m particle size) with
mobile phase consisting of acetonitrile and 0.05M
phosphate buffer in the ratio of 25:75 v/v in
isocratic mode was used. The detection wavelength
is 293nm and the flow rate is 0.8ml/min.
Ciprofloxacin is used as internal standard. The
linearity was found to be in the range of 0.1 to 10
g/ml and shows a correlation coefficient of
0.9999. The intra- and inter-day variation was
found to be less than 1.2% showing high precision
of the assay method. The mean percent recovery of
the drug from the solution containing 4 g/ml was
100.5 2.06 indicating high accuracy of the
proposed method. Hence, this method is simple,
fast, specific, accurate, precise and less time
consuming for the estimation of gatifloxacin in
pharmaceutical dosage forms


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