ANTIBACTERIAL EFFICACY OF VARIETY PLANTS AGAINST THE RESISTANT STREPTOCOCCUS WHICH CAUSE CLINICAL MASTITIS IN COWS

SHAZA ANWAR AL LAHAM, FRDOOS MOHAMMAD AL FADEL

Abstract


Streptococcus is considered to be one of the most dangerous causes of Clinical mastitis in cows. The aim of this study was to investigate the effectiveness of extracts prepared from different parts of the flowing plants: Oleaeuropea Linn(Oleaceae), Myrtuscommunis Linn (Liliaceae) ,thymus vulgaris Linn (Laminaceae), Rosemery Linn(Laminaceae), Ficuscarica Linn (Moraceae), and Achilleafalcata Linn(Asteraceae) against resistant Streptococcus in 1371 Samples of milk. This work was achieved in four stages: First of all, the presence of Streptococcus in 1371 Samples of milk, by using blood agar, Bile Esculin agar, and some bio-chemical tests were investigated .Secondly, the antibacterial activity of many antibiotics on these bacteria by using disc diffusion method were determined.Thirdly, the plants were extracted with water, absolute alcohol, and petroleum Ether by using soxhlet apparatus and rotary vacuum evaporator. Fourthly, the antibacterial activity of the extractions on resistant Streptococcus was determined by using disc diffusion method. This study has shown the presence of different antibacterial effectiveness of the extracts prepared from different parts of those plants. The extract of thymus vulgaris is more effective when compared to the extract of Oleaeur opaea against resistant Streptococcus.

 


Full Text:

PDF

References


Hosainzadegan H, Alizadeh M, Karimi F, Pakzad P. Study of antibacterial effects of ripped and raw fig alone and in combination. Journal of Medicinal Plants Research. 2012;6(14):2864-7.

Belaj A, Satovic Z, Rallo L, Trujillo I. Genetic diversity and relationships in olive (Olea europaea L.) germplasm collections as determined by randomly amplified polymorphic DNA. TAG Theoretical and Applied Genetics. 2002;105(4):638-44.

Pereira AP, Ferreira IC, Marcelino F, Valentão P, Andrade PB, Seabra R, et al. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Molecules. 2007;12(5):1153-62.

Gruenwald J, Brendler T, Jaenicke C. Physicians' Desk Reference(PDR) For Herbal Medicines: Medical Economics Company; 2000.

Al-Anbori DK, Al-Nimer MS, Al-Weheb AM. Athraa M:Antibacterial activity of ethanolic extract of Myrtus communis. L leaves against salivary Mutans streptococci. Saudi Dental Journal. 2008;20(3):82-7.

Qaralleh HN, ABBOUD MM, KHLEIFAT KM, Tarawneh K, Althunibat O. Antibacterial activity in vitro of Thymus capitatus from Jordan. Pak J Pharm Sci. 2009;22(3):247-51.

Bozin B, Mimica-Dukic N, Samojlik I, Jovin E. Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. Journal of agricultural and food chemistry. 2007;55(19):7879-85.

Karaalp C, Yurtman AN, Karabay Yavasoglu NU. Evaluation of antimicrobial properties of Achillea L. flower head extracts. Pharmaceuticalbiology. 2009;47(1):86-91.

Martín BS, Kruze J, Morales MA, Agüero H, Iragüen D, Espinoza S, et al. Antimicrobial Resistance in Bacteria Isolated From Dairy Herds in Chile. The International Journal of Applied Research in Veterinary Medicine. 2009;4(7)

Radulović N, Stankov-Jovanović V, Stojanović G, Šmelcerović A, Spiteller M, Asakawa Y. Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans. Food chemistry. 2007;103(1):15-21.

Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology. 2006;17(6):300-12.

Hudzicki J. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. 2009.

Wilker MA, Cockerill FR, Craig WA, Dudley MN, Eliopoulos GM, Hecht DW. M100-S17 Performance Standards for Antimicrobial Susceptibility Testing;Seventeenth Informationl Supplement. 2007;27(1).

Kayser FH. Medical microbiology: Georg Thieme Verlag; 2005.

Ibrahim S, alRifai H. The role of Staphylococcus and Streptococcus in the incidence of mastitis in sheep in the central region in Syria. the Baath Journal. 2009;31(4).

Ibrahim S, alRifai H. The role of Staphylococcus and Streptococcus in the incidence of mastitis in sheep in the central region in Syria. Journal of the Baath. 2009;31(4).

Cheng DR, Zhu SY, Yin ZH, Ding WW, Mu ZX, Su ZR, et al. Prevalence of bacterial infection responsible for bovine mastitis. African Journal of MicrobiologyResearch. 2010;4(11):1110-6.

Sumathi B, Veeregowda B, Amitha RG. Prevalence and antibiogram profile of bacterial Isolates from clinical bovine mastitis. Veterinary World. 2008;1(8):237-8.

Myllys V. Staphylococci in heifer mastitis before and afterparturition. Journal of Dairy Research. 1995;62(01):51-60.

Atyabi N, Vodjgani M, Gharagozloo F, Bahonar A. Prevalence of bacterial mastitis in cattle from the farms around Tehran. Iranian J Vet Res. 2006;7:76-9.

Al-Juraifani AA. Antimicrobial activity of some medicinal plants used in Saudi Arabia. Canadian journal of pure and applied sciences. 2009:1509.

González M, Marioli J. Antibacterial activity of water extracts and essential oils of various aromatic plants against Paenibacillus larvae, the causative agent of American Foulbrood. Journal of invertebrate pathology. 2010;104(3):209-13.

Ivanovic J, Misic D, Zizovic I, Ristic M. In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. Food Control. 2011.

Rota MC, Herrera A, Martínez RM, Sotomayor JA, Jordán MJ. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food Control. 2008;19(7):681-7.

Silva N, Fernandes Júnior A. Biological properties of medicinal plants: a review of their antimicrobial activity. J Venom Anim Toxins incl Trop Dis. 2010;16(3):402-13.

Oyeleke S, Dauda B, Boye O. Antibacterial activity of Ficus capensis. African Journal of Biotechnology. 2010;7(10).

Mandal SC, Saha B, Pal M. Studies on antibacterial activity of Ficus racemosa Linn. leaf extract. Phytotherapy Research. 2000;14(4):278-80.

Ali NH, Faizi S, Kazmi SU. Antibacterial activity in spices and local medicinal plants against clinical isolates of Karachi, Pakistan. Pharm Biol. 2011 Aug;49(8):833-9.

Moreno S, Scheyer T, Romano CS, Vojnov AA. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. FreeRadical Research. 2006;40(2):223-31.

Tsai PJ, Tsai TH, Ho SC. In vitro inhibitory effects of rosemary extracts on growth and glucosyltransferase activity of Streptococcus sobrinus. Food chemistry. 2007;105(1):311-6.

Amensour M, Bouhdid S, Fernández-López J, Idaomar M, Senhaji NS, Abrini J. Antibacterial activity of extracts of Myrtus communis against food-borne pathogenic and spoilage bacteria. International Journal of Food Properties. 2010;13(6):1215-24.

Khder AK. Effect of Allium sativum and Myrtus communis on the elimination of antibiotic resistance and swarming of Proteus mirabilis. Jordan Journal of Biological Sciences (JJBS). 2008:3124.

Mansouri S, Foroumadi A, Ghaneie T, Najar AG. Antibacterial activity of the crude extracts and fractionated constituents of Myrtus communis. Pharmaceutical biology. 2001;39(5):399-401.

Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sökmen A, et al. Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp millefolium Afan.(Asteraceae). Journal of Ethnopharmacology. 2003;87(2):215-20.

Stojanović G, Radulović N, Hashimoto T, Palić R. In vitro antimicrobial activity of extracts of four Achillea species: The composition of Achillea clavennae L.(Asteraceae) extract. Journal of ethnopharmacology. 2005;101(1):185-90.

Omar SH. Oleuropein in olive and its pharmacological effects. Scientia pharmaceutica. 2010;78(2):133.

H, Alizadeh M, Karimi F, Pakzad P. Study of antibacterial effects of ripped and raw fig alone and in combination. Journal of Medicinal Plants Research. 2012;6(14):2864-7.


Refbacks

  • There are currently no refbacks.




 

 

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

 

Listed as Green Publisher

Powered by Informatics India Ltd