SCREENING THE ANTIMICROBIAL ACTIVITY OF SOME MEDICINAL PLANTS AGAINST MULTIDRUG RESISTANCE ESCHERICHIA COLI TYPE (1)

SHAZA ANWAR AL LAHAM, FRDOOS MOHAMMAD

Abstract


The increasing number of Escherichia coli causing mastitis and of bacteria resistant to conventional antibiotics has become a
serious problem in recent years. So the search for new antibiotics and alternative products to solve this problem is the question of
the age. This research aims to investigate the effectiveness of the extracts prepared from different parts of the following plants:
Olea europea Linn (Oleaceae) ، Myrtus communis Linn (Liliaceae)، Majorana syriacus Linn (Laminaceae)، Zingiber officinale
Linn (Zingiberaceae)، Achillea falcata Linn (Asteraceae) against resistant Escherichia coli Type (1). Investigation began for
E.coli bacteria in 667 milk samples. The bacteria were identified culturally, morphologically and biochemically. Antibiotic
susceptibility testing against E.coli by Kirby-Bauer disk diffusion method were conducted. Then using the blood agar,
MacConkey agar, salmonella - shigella agar, and biochemical testing method [API 20 E testing Enterobacteriaceae] were made to
type E.coli. Plants were extracted with water, absolute alcohol, then ether using a soxhlet apparatus and rotary vacuum
evaporator. Then extracts susceptibility testing against antibiotic resistant E.coli Type (1) were studied. E. coli was defined as
oxidase negative, indole positive, catalase positive. The studied antibiotics did not show any antibacterial effect against E.coli .
By the results of the biochemical analysis (API20e) on resistant E.coli , E.coli type (1) was 33.35% of the total number of
samples. The anti-bacterial effectiveness against E.coli type (1) of ethanol extracts prepared from different parts of the studied
plants were variant, whereas the Myrtus communis extract effectively has the most powerful antibacterial effect for these bacteria,
while the Zingiber officinale extract has the lowest influence.


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