ACTINOMYCIN “D” FROM MARINE SEDIMENT ASSOCIATED STREPTOMYCES CAPILLISPIRALIS MTCC10471

M.SRINU1, M. MURALI KRISHNA KUMAR, G. GIRIJA SHANKAR

Abstract


In our screening program for new bio-active metabolites from marine actinomycetes, a cyclic depsipeptide was
found in the fermentation medium of marine Strepromyces (SS23/4) isolated from sediments collected from Bay
off Bengal, vellampattai,Tamilnadu. It showed strong biological activity against gram-positive / gram negative
bacteria by agar overlay technique. It was taxonomically characterized by the basis of morphological and
phenotypic characteristics, genotypic data and phylogenetic showing Streptomyces sps. Bio active compound
was obtained by solvent extraction and purification using column chromatography followed by reverse phase
HPLC. The pure compound had potent activity against Mycobacterium tuberculosis and Multi Drug Resistant
Mycobacterium tuberculosis strains (437RU) at a concentration of 10 μg/mL, and The minimum inhibitory
concentration (MIC) against standard test organisms was found to be 1μg/mL against B.subtilis, E.coli and
Methicillin resistant Staphylococcus aureus. The compound exhibited potent cytotoxic activity against breast
carcinoma (MCF-7), melanoma cells (A375), prostate carcinoma (DU145) and lung carcinoma (A549) cells
with IC values 20μg/ml. The symbiotic Streptomyces capillispiralis MTCC 10471 produces crude antibiotic
30mg/Lt by using nonoptimized fermentation conditions. The structure of the antibiotic was explained by 1D,
2D NMR and LC-ESI-MS/MS, MALDI-TOF/MS experiments, revealed that it belongs to cyclic ploy peptide
Actinomycin D.


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