HPLC DETERMINATION OF PARTHENOLIDE AND APIGENIN CONTENTS VERSUS LABEL CLAIMS IN IRANIAN PRODUCTS

GHAFARI S, ESMAEILI S NAGHIBI F, MOSADDEGH M

Abstract


In this study parthenolide content of feverfew product (Tanamigraine capsule) and apigenin content of chamomile products (Chamomill drop, Kamisol solution, Prostatan drop, Prostatan tablet) were determined as effective compound by HPLC. Feverfew product was extracted by methanol/distilled de-ionized water (50: 50) and chamomile products by methanol. The HPLC operating conditions were C-18 reversed phase column (VP-ODS 250×4.6 mm, 5 mm), methanol/ distilled de-ionized water (60: 40) as the mobile phase at a flow rate of 1 ml/min and UV detection at 214 nm for parthenolide and 2% acetic acid: acetonitril with linear program 80 to 40 % of acetic acid 2% with a flow rate of 1 ml/min and UV detection at 275nm for apigenin. The amount of parthenolide and apigenin obtained in Tanamigraine capsules and chamomile products is 0.13- 016% and 0.04- 35% respectively

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