NEW SPECTROPHOTOMETRIC METHODS DEVELOPMENT FOR THE DETERMINATION OF OSELTAMIVIR PHOSPHATE IN CAPSULES BASED ON THE OXIDATION REACTIONS OF THE OLEFENIC DOUBLE BOND
Simple, sensitive and selective spectrophotometric methods (M1 and M2) for the assay of oseltamivir phosphate (OP) through the olefenic double bond are proposed. Method M1 is based on the reaction of potassium permanganate to the olefenic double bond in OP and estimating the unreacted permanganate with fast green FCF (FGFCF). Method M2 involves the treatment of the olefenic double bond in OP with a Lemieux reagent (mixture of KMnO4 and NaIO4) and estimating the aldehyde formed with 3-methyl-2-benzothiazolinone hydrazone (MBTH). The color produced in M1 and M2 methods has maximum absorption at 620nm and 654nm respectively. Beer’s law obeyed in the concentration range of 4-20μg/ml and 4-12 μg/ml for method M1 and M2 respectively. The precision and accuracy of the methods are checked by the reported UV reference method. No interference was observed from the usually existing additives in pharmaceutical formulations and the applicability of the methods was examined by analyzing NATFLUE capsules containing OP. The both methods are found to be suitable for the determination of oseltamivir phosphate.
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