TOTAL PHENOLICS, TOTAL TANNINS AND ANTIOXIDANT ACTIVITY OF CASSIA FISTULA L. EXTRACTS OF BARK, STEM, LEAF AND ROOT UNDER DIFFERENT AGE CLASSES

LAI TZEKIAT, LIEW KANG CHIANG

Abstract


Indian Laburnum (Cassia fistula L.) bark, stem, leaf and root under different age classes namely Class A (2-3
years), Class B (5-10 years) and Class C (10-15 years) were analyzed for their total phenolic content (TPC),
total tannin content (TTC) and antioxidant activity (AA). Both total phenolic content (TPC) and total tannin
content (TTC) were determined using Folin-Ciocalteau assays and antioxidant activity (AA) was carried out by
Free Radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. From the study, higher TPC, TTC and AA were
observed in Cassia fistula bark extracts compare to otherportion extracts (stem, leaf and root). Bark extracts
from three different age classes, showed total means of 16.67 % TPC and 3.12% TTC. In addition, bark extracts
from three different age classes also showed high antioxidant activity (AA) with mean IC50 values of 0.04g/ml.


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