Screening of some Naturally Isolated Microalgal Strains for Polyunsaturated Fatty Acids Production

Mohammad Hossein Morowvat, Younes Ghasemi


Background and Purpose: Nowadays, polyunsaturated fatty acids (PUFAs) are playing a great role in human wellbeing and health improvement. A wide spectrum of biological, medical and health benefit effects ranging from cardiovascular, neuronal, anticancer and antioxidant have been reported from different PUFAs in human. Methodology: In this study, six different species of microalgae belonging to the chlorophyta and cyanobacteria phylum were isolated from soil and water samples collected from Persian Gulf. Their growth rate, biomass and lipid production and productivity and more importantly their ability to produce PUFAs was investigated. Results: The isolated species represented a great fatty acid profile including many different polyunsaturated fatty acids (PUFAs) ranging from 6-20 carbon atoms. S. obliquus and N. muscorum proven to have a better profile for PUFAs production, whilst C. vulgaris could be considered as a more robust strain to produce other fatty acid classes. Besides, C. vulgaris with its higher growth rates (0.39 d-1)and S. obliquus owing to its higher total lipid content (43.92%) seems more interesting strains for scale up studies. Conclusion: The obtained results demonstrated the great potential of naturally isolated strains of microalgae for PUFA production and provided some insights in next studies to explore more producing strains.

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Gill I, Valivety R. Polyunsaturated fatty acids, part 1: Occurrence,

biological activities and applications. Trends in

Biotechnology. 1997; 15(10):401–9.

Auestad N, Scott DT, Janowsky JS, Jacobsen C, Carroll RE,

Montalto MB, et al. Visual, cognitive, and language assessments

at 39 months: a follow-up study of children fed formulas

containing long-chain polyunsaturated fatty acids to

year of age. Pediatrics. 2003; 112(3 Pt 1):e177–83.

Fleith M, Clandinin MT. Dietary PUFA for preterm and

term infants: review of clinical studies. Critical Reviews in

Food Science and Nutrition. 2005; 45(3):205–29.

Alessandri JM, Guesnet P, Vancassel S, Astorg P, Denis I,

Langelier B, et al. Polyunsaturated fatty acids in the central

nervous system: evolution of concepts and nutritional implications

throughout life. Reproduction, Nutrition, Development.

; 44(6):509–38.

Brenna JT, Diau GY. The influence of dietary docosahexaenoic

acid and arachidonic acid on central nervous system

polyunsaturated fatty acid composition. Prostaglandins, Leukotrienes,

and Essential Fatty Acids. 2007; 77(5-6):247–50.

Das UN. Folic acid and polyunsaturated fatty acids improve

cognitive function and prevent depression, dementia, and

Alzheimer’s disease-but how and why? Prostaglandins,

Leukotrienes, and Essential Fatty Acids. 2008; 78(1):11–9.

Mullen A, Loscher CE, Roche HM. Anti-inflammatory

effects of EPA and DHA are dependent upon time and

dose-response elements associated with LPS stimulation in

THP-1-derived macrophages. The Journal of Nutritional

Biochemistry. 2010; 21(5):444–50.

Hirano M, Mori H, Miura Y, Matsunaga N, Nakamura

N, Matsunaga T. γ-linolenic acid production by microalgae.

Applied Biochemistry and Biotechnology. 1990; 24-


Nichols BW, Appleby RS. The distribution and biosynthesis

of arachidonic acid in algae. Phytochemistry. 1969;


Guedes AC, Amaro HM, Barbosa CR, Pereira RD, Malcata

FX. Fatty acid composition of several wild microalgae and

cyanobacteria, with a focus on eicosapentaenoic, docosahexaenoic

and α-linolenic acids for eventual dietary uses.

Food Research International. 2011; 44(9):2721–9.

Ghasemi Y, Rasoul-Amini S, Morowvat MH. Algae for the

production of SCP. In: Liong MT, editor. Bioprocess Sciences

and Technology: Nova Science Publishers, Inc. 2011. p.


De Swaaf ME, Sijtsma L, Pronk JT. High-cell-density fedbatch

cultivation of the docosahexaenoic acid producing

marine alga Crypthecodinium cohnii. Biotechnology and

Bioengineering. 2003; 81(6):666–72.

Guil-Guerrero JL, Belarbi EIH, Rebolloso-Fuentes MM.

Eicosapentaenoic and arachidonic acids purification from

the red microalga Porphyridium cruentum. Bioseparation.

; 9(5):299–306.

Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial

applications of microalgae. Journal of Bioscience

and Bioengineering. 2006; 101(2):87–96.

Tonon T, Harvey D, Larson TR, Graham IA. Long chain

polyunsaturated fatty acid production and partitioning to

triacylglycerols in four microalgae. Phytochemistry. 2002;


Khozin-Goldberg I, Iskandarov U, Cohen Z. LC-PUFA

from photosynthetic microalgae: Occurrence, biosynthesis,

and prospects in biotechnology. Applied Microbiology and

Biotechnology. 2011; 91(4):905–15.

Ghasemi Y, Faramarzi MA, Arjmand-Inalou M, Mohagheghzadeh

A, Shokravi S, Morowvat MH. Side-chain

cleavage and C-20 ketone reduction of hydrocortisone by a

natural isolate of Chroococcus dispersus. Annals of Microbiology.

; 57(4):577–81.

Ghasemi Y, Rasoul-Amini S, Morowvat MH, Raee MJ, Ghoshoon

MB, Nouri F, et al. Characterization of hydrocortisone

biometabolites and 18S rRNA gene in Chlamydomonas

reinhardtii cultures. Molecules. 2008;13(10):2416–25.

Shaker S, Nemati A, Montazeri-Najafabady N, Mobasher

MA, Morowvat MH, Ghasemi Y. Treating urban wastewater:

Nutrient removal by using immobilized green algae in

batch cultures. International Journal of Phytoremediation.

; 17(12):1177–82.

Ghasemi Y, Mohagheghzadeh A, Moshavash M, Ostovan Z,

Rasoul-Amini S, Morowvat MH, et al. Biotransformation of

monoterpenes by Oocystis pusilla. World Journal of Microbiology

and Biotechnology. 2009; 25(7):1301–4.

Ghasemi Y, Rasoul-Amini S, Kazemi A, Zarrinic G, Morowvat

MH, Kargar M. Isolation and characterization of

some moderately halophilic bacteria with lipase activity.

Mikrobiologiia. 2011; 80(4):477–81.

Ghasemi Y, Rasoul-Amini S, Morowvat MH, Azam SBM,

Shokravi S, Mohagheghzadeh A, et al. Bioconversion of hydrocortisone

by unicellular microalga Oocystis pusilla. Biotechnology.

; 7(2):293–8.

Morowvat MH, Rasoul-Amini S, Ghasemi Y. Chlamydomonas

as a “new” organism for biodiesel production. Bioresource

Technology. 2010; 101(6):2059–62.

Rasoul-Amini S, Ghasemi Y, Morowvat MH, Mohagheghzadeh

A. PCR amplification of 18S rRNA, single cell protein

production and fatty acid evaluation of some naturally isolated

microalgae. Food Chemistry. 2009; 116(1):129–36.

Lee E, Jalalizadeh M, Zhang Q. Growth kinetic models

for microalgae cultivation: A review. Algal Research.


Ghasemi Y, Khalaj A, Mohagheghzadeh A, Khosravi AR,

Morowvat MH. Composition and antimicrobial activity of

the essential oil and extract of Hypericum elongatum. Journal

of Applied Sciences. 2007; 7(18):2671–5.

Lang I, Hodac L, Friedl T, Feussner I. Fatty acid profiles and

their distribution patterns in microalgae: A comprehensive

analysis of more than 2000 strains from the SAG culture

collection. BMC Plant Biology. 2011; 11(1):1–16.

Griffiths MJ, Harrison STL. Lipid productivity as a key characteristic

for choosing algal species for biodiesel production.

Journal of Applied Phycology. 2009; 21(5):493–507.

Xue Z, Sharpe PL, Hong S-P, Yadav NS, Xie D, Short DR, et

al. Production of omega-3 eicosapentaenoic acid by metabolic

engineering of Yarrowia lipolytica. Nature Biotechnology.

; 31(8):734–40.

Xie D, Jackson EN, Zhu Q. Sustainable source of omega-3

eicosapentaenoic acid from metabolically engineered Yarrowia

lipolytica: from fundamental research to commercial

production. Applied Microbiology and Biotechnology.

; 99:1599–610.


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