Screening of some Naturally Isolated Microalgal Strains for Polyunsaturated Fatty Acids Production
Background and Purpose: Nowadays, polyunsaturated fatty acids (PUFAs) are playing a great role in human wellbeing and health improvement. A wide spectrum of biological, medical and health benefit effects ranging from cardiovascular, neuronal, anticancer and antioxidant have been reported from different PUFAs in human. Methodology: In this study, six different species of microalgae belonging to the chlorophyta and cyanobacteria phylum were isolated from soil and water samples collected from Persian Gulf. Their growth rate, biomass and lipid production and productivity and more importantly their ability to produce PUFAs was investigated. Results: The isolated species represented a great fatty acid profile including many different polyunsaturated fatty acids (PUFAs) ranging from 6-20 carbon atoms. S. obliquus and N. muscorum proven to have a better profile for PUFAs production, whilst C. vulgaris could be considered as a more robust strain to produce other fatty acid classes. Besides, C. vulgaris with its higher growth rates (0.39 d-1)and S. obliquus owing to its higher total lipid content (43.92%) seems more interesting strains for scale up studies. Conclusion: The obtained results demonstrated the great potential of naturally isolated strains of microalgae for PUFA production and provided some insights in next studies to explore more producing strains.
Gill I, Valivety R. Polyunsaturated fatty acids, part 1: Occurrence,
biological activities and applications. Trends in
Biotechnology. 1997; 15(10):401–9.
Auestad N, Scott DT, Janowsky JS, Jacobsen C, Carroll RE,
Montalto MB, et al. Visual, cognitive, and language assessments
at 39 months: a follow-up study of children fed formulas
containing long-chain polyunsaturated fatty acids to
year of age. Pediatrics. 2003; 112(3 Pt 1):e177–83.
Fleith M, Clandinin MT. Dietary PUFA for preterm and
term infants: review of clinical studies. Critical Reviews in
Food Science and Nutrition. 2005; 45(3):205–29.
Alessandri JM, Guesnet P, Vancassel S, Astorg P, Denis I,
Langelier B, et al. Polyunsaturated fatty acids in the central
nervous system: evolution of concepts and nutritional implications
throughout life. Reproduction, Nutrition, Development.
Brenna JT, Diau GY. The influence of dietary docosahexaenoic
acid and arachidonic acid on central nervous system
polyunsaturated fatty acid composition. Prostaglandins, Leukotrienes,
and Essential Fatty Acids. 2007; 77(5-6):247–50.
Das UN. Folic acid and polyunsaturated fatty acids improve
cognitive function and prevent depression, dementia, and
Alzheimer’s disease-but how and why? Prostaglandins,
Leukotrienes, and Essential Fatty Acids. 2008; 78(1):11–9.
Mullen A, Loscher CE, Roche HM. Anti-inflammatory
effects of EPA and DHA are dependent upon time and
dose-response elements associated with LPS stimulation in
THP-1-derived macrophages. The Journal of Nutritional
Biochemistry. 2010; 21(5):444–50.
Hirano M, Mori H, Miura Y, Matsunaga N, Nakamura
N, Matsunaga T. γ-linolenic acid production by microalgae.
Applied Biochemistry and Biotechnology. 1990; 24-
Nichols BW, Appleby RS. The distribution and biosynthesis
of arachidonic acid in algae. Phytochemistry. 1969;
Guedes AC, Amaro HM, Barbosa CR, Pereira RD, Malcata
FX. Fatty acid composition of several wild microalgae and
cyanobacteria, with a focus on eicosapentaenoic, docosahexaenoic
and α-linolenic acids for eventual dietary uses.
Food Research International. 2011; 44(9):2721–9.
Ghasemi Y, Rasoul-Amini S, Morowvat MH. Algae for the
production of SCP. In: Liong MT, editor. Bioprocess Sciences
and Technology: Nova Science Publishers, Inc. 2011. p.
De Swaaf ME, Sijtsma L, Pronk JT. High-cell-density fedbatch
cultivation of the docosahexaenoic acid producing
marine alga Crypthecodinium cohnii. Biotechnology and
Bioengineering. 2003; 81(6):666–72.
Guil-Guerrero JL, Belarbi EIH, Rebolloso-Fuentes MM.
Eicosapentaenoic and arachidonic acids purification from
the red microalga Porphyridium cruentum. Bioseparation.
Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial
applications of microalgae. Journal of Bioscience
and Bioengineering. 2006; 101(2):87–96.
Tonon T, Harvey D, Larson TR, Graham IA. Long chain
polyunsaturated fatty acid production and partitioning to
triacylglycerols in four microalgae. Phytochemistry. 2002;
Khozin-Goldberg I, Iskandarov U, Cohen Z. LC-PUFA
from photosynthetic microalgae: Occurrence, biosynthesis,
and prospects in biotechnology. Applied Microbiology and
Biotechnology. 2011; 91(4):905–15.
Ghasemi Y, Faramarzi MA, Arjmand-Inalou M, Mohagheghzadeh
A, Shokravi S, Morowvat MH. Side-chain
cleavage and C-20 ketone reduction of hydrocortisone by a
natural isolate of Chroococcus dispersus. Annals of Microbiology.
Ghasemi Y, Rasoul-Amini S, Morowvat MH, Raee MJ, Ghoshoon
MB, Nouri F, et al. Characterization of hydrocortisone
biometabolites and 18S rRNA gene in Chlamydomonas
reinhardtii cultures. Molecules. 2008;13(10):2416–25.
Shaker S, Nemati A, Montazeri-Najafabady N, Mobasher
MA, Morowvat MH, Ghasemi Y. Treating urban wastewater:
Nutrient removal by using immobilized green algae in
batch cultures. International Journal of Phytoremediation.
Ghasemi Y, Mohagheghzadeh A, Moshavash M, Ostovan Z,
Rasoul-Amini S, Morowvat MH, et al. Biotransformation of
monoterpenes by Oocystis pusilla. World Journal of Microbiology
and Biotechnology. 2009; 25(7):1301–4.
Ghasemi Y, Rasoul-Amini S, Kazemi A, Zarrinic G, Morowvat
MH, Kargar M. Isolation and characterization of
some moderately halophilic bacteria with lipase activity.
Mikrobiologiia. 2011; 80(4):477–81.
Ghasemi Y, Rasoul-Amini S, Morowvat MH, Azam SBM,
Shokravi S, Mohagheghzadeh A, et al. Bioconversion of hydrocortisone
by unicellular microalga Oocystis pusilla. Biotechnology.
Morowvat MH, Rasoul-Amini S, Ghasemi Y. Chlamydomonas
as a “new” organism for biodiesel production. Bioresource
Technology. 2010; 101(6):2059–62.
Rasoul-Amini S, Ghasemi Y, Morowvat MH, Mohagheghzadeh
A. PCR amplification of 18S rRNA, single cell protein
production and fatty acid evaluation of some naturally isolated
microalgae. Food Chemistry. 2009; 116(1):129–36.
Lee E, Jalalizadeh M, Zhang Q. Growth kinetic models
for microalgae cultivation: A review. Algal Research.
Ghasemi Y, Khalaj A, Mohagheghzadeh A, Khosravi AR,
Morowvat MH. Composition and antimicrobial activity of
the essential oil and extract of Hypericum elongatum. Journal
of Applied Sciences. 2007; 7(18):2671–5.
Lang I, Hodac L, Friedl T, Feussner I. Fatty acid profiles and
their distribution patterns in microalgae: A comprehensive
analysis of more than 2000 strains from the SAG culture
collection. BMC Plant Biology. 2011; 11(1):1–16.
Griffiths MJ, Harrison STL. Lipid productivity as a key characteristic
for choosing algal species for biodiesel production.
Journal of Applied Phycology. 2009; 21(5):493–507.
Xue Z, Sharpe PL, Hong S-P, Yadav NS, Xie D, Short DR, et
al. Production of omega-3 eicosapentaenoic acid by metabolic
engineering of Yarrowia lipolytica. Nature Biotechnology.
Xie D, Jackson EN, Zhu Q. Sustainable source of omega-3
eicosapentaenoic acid from metabolically engineered Yarrowia
lipolytica: from fundamental research to commercial
production. Applied Microbiology and Biotechnology.
- There are currently no refbacks.
Listed as Green Publisher